
Thermo Fisher Scientific AHA1 Monoclonal Antibody (25F2.D9), APC
AHA1 단백질을 인식하는 Rat 유래 Monoclonal 항체로 APC 형광 표지. Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Protein G 정제, 4°C 보관.
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Thermo Fisher Scientific AHA1 Monoclonal Antibody (25F2.D9), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25F2.D9 |
| Immunogen | Recombinant Full Length Mouse Aha1 Protein |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931687 |
Additional Formats
- Unconjugated: MA5-27641
- FITC: MA5-45234
- PE: MA5-45236
- PerCP: MA5-45235
- Request custom conjugation available
Product Specific Information
1 µg/mL of MA5-45233 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Detects approximately 38 kDa, can run up to 45 kDa on SDS-PAGE.
Target Information
Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1, increasing its chaperone activity. Competes with inhibitory co-chaperones FNIP1 and TSC1 for binding to HSP90AA1, providing a reciprocal regulatory mechanism for chaperoning of client proteins. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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