Thermo Fisher Scientific Human Fc gamma RIIIA (CD16a) (176 Val) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD16a, amino acids Met1-Gln208 (Accession # P08637-1) with natural variant F176V with a C-terminal His-tag

Tag

His-tag

Molecular weight

23.3 kDa

Class

Recombinant

Type

Protein

Purity

>95 % as determined by SDS-PAGE. >95 % as determined by SEC-HPLC

Endotoxin concentration

<1.0 EU/µg

Activity

1. Loaded Human FcγRIIA / CD16a (V176) recombinant protein on His1K Biosensor, can bind Bevaciizumab (IgG1) with an affinity constant of 0.83 µM as determined in a BLI assay.

2. Captured Human FcγRIIA / CD16a (V176) recombinant protein on Anti-His chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 µM as determined in a SPR assay.

3. Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIA / CD16a (V176) recombinant protein with an affinity constant of 0.78 µM as determined in an SPR assay.

4. Loaded Recombinant Human FcγRIIA / CD16a (V176) Protein, His Tag on His1K Biosenseor, can bind Bevacizumab (IgG1) with an affinity constant of 0.81 µM as determined in BLI assay.

5. Loaded Rituximab (IgG1) on proA Biosensor, can bind Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein, His Tag with an affinity constant of 0.622 µM as determined in BLI assay.

6.Loaded Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein, His Tag on HIS1K Biosensor, can bind Rituximab with an affinity constant of 0.447 µM as determined in BLI assay.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.