Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), Brilliant Ultra Violet 805, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395, 496, 615, 737
• Brilliant Violet 605, 650
• eFluor 450, 506
• NovaFluor Blue / Red / Yellow Series
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436~780
• Request custom conjugation available

Product Specific Information

The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except plasma cells. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associated with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation. The SJ25C1 antibody and HIB19 recognize overlapping epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.5 µg)/test.
Cell number range: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is compatible with ultraviolet (355 nm) laser systems. Ensure instrument compatibility.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
BUV805 is compatible with short-term methanol-based fixation (<1 hour).

Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR) and plays a key role in regulating normal and malignant B cell proliferation. Mutation of CD19 leads to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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