Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor series (Blue, Red, UV, Violet, Yellow)
• PE, PE-Cyanine5/5.5/7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells at all developmental stages except plasma cells. CD19 forms a multimolecular complex with CD21, CD81, and MHC class II, which associates with the BCR to regulate B cell signaling and proliferation.

• Reported Applications: Flow cytometric analysis
• Tested Applications: Mouse splenocytes; ≤0.5 µg/test (100 µL final volume)
• Recommended: Titrate for optimal performance

Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and requires a UV (355 nm) laser-equipped cytometer. Protect from light to avoid photo-induced oxidation.

Staining and Fixation

• Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to reduce non-specific polymer interactions.
• Samples can be fixed using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
• Compatible with short-term methanol fixation; avoid prolonged methanol exposure.

Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and certain myeloid leukemia cells. CD19 plays a crucial role in B cell receptor signaling, proliferation, and differentiation. Mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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