Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 480
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with a molecular weight of 50–80 kDa. CD16 includes two genes: CD16A (FcγRIIIA), expressed in macrophages and NK cells, and CD16B (FcγRIIIB), expressed in neutrophils. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, initiating signal transduction leading to ADCC, phagocytosis, and degranulation.

Applications Reported:
This antibody is suitable for flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Use 5 µL (0.125 µg) per test in 100 µL final volume. Compatible with 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is intended for UV (355 nm) laser-equipped cytometers. Protect from light due to photo-induced oxidation sensitivity.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific interactions.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark. Compatible with short-term methanol fixation (<1 hour).

Excitation / Emission / Laser:
355 nm / 797 nm / Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD16 encodes a receptor recognizing the Fc portion of IgG, participating in immune complex clearance, ADCC, and modulation of viral infection susceptibility.
The gene FCGR3A is expressed on NK cells (transmembrane form), while FCGR3B is expressed on neutrophils (GPI-linked form). Mutations are associated with immunodeficiency 20, systemic lupus erythematosus, and neonatal neutropenia.

For Research Use Only.
Not for diagnostic procedures or resale without authorization.

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