Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Biotin
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein expressed on resting conventional B cells and modulated upon B-cell activation. CD23 acts as a low-affinity receptor for IgE and plays a role in IgE regulation and B-cell activation. CD21 is thought to bind CD23.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤1.0 µg/test
  (A test is defined as the amount of antibody staining a sample in 100 µL volume; cell number range: 10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is intended for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility.

When using multiple polymer dye–conjugated antibodies, use:

• Super Bright Complete Staining Buffer (SB-4401)
• Brilliant Stain Buffer (00-4409-75)

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
Methanol Compatibility: Short-term methanol fixation is compatible; avoid long-term storage in methanol-containing buffers (>1 hour).

Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD23 is a 45 kDa glycoprotein expressed on a subset of peripheral blood and tonsil B cells, and strongly on EBV-transformed B lymphoblasts. It is identical to the low-affinity IgE receptor on B cells. CD23 expression is observed in B-cell chronic lymphocytic leukemia and some lymphomas. Functionally, CD23 regulates B-cell growth, differentiation, and IgE production. Soluble CD23 acts as a mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures.

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