Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 506
• FITC
• NovaFluor Blue / Red / Violet / Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436–780)
• Custom conjugation available

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). CD11b is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It acts as a receptor for ICAM family members (CD54, CD102, CD50) and iC3b, playing a crucial role in cell-cell and cell-matrix interactions.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry on human peripheral blood cells (5 µL/test)

Brilliant Ultra Violet™ 805 (BUV805):

• Emission: 797 nm
• Excitation: 355 nm (UV laser required)
• Light-sensitive; protect from light
• Compatible with short-term methanol fixation (≤1 hr)
• Use Brilliant Stain Buffer or Super Bright Staining Buffer to minimize non-specific interactions
• Fixation: IC Fixation Buffer or 1-step Fix/Lyse Solution can be used for up to 3 days at 4°C in the dark

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimeric complex with integrin beta-2 (CD18), known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. Expression varies by tissue type and cell maturation stage. CD11b is a known microglial marker and is implicated in systemic lupus erythematosus susceptibility.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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