Thermo Fisher Scientific RAE1 delta Monoclonal Antibody (RD-41), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Flow Cytometry (Flow)

0.25 µg/test

View 2 publications 2 publications

ELISA (ELISA)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RD-41

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210509

Product Specific Information

Description: The RD-41 monoclonal antibody reacts with the murine Rae1delta. Rae-1delta is one of several known murine NKG2D ligands that include RAE-1 molecules (alpha, beta, ε, gamma and delta), H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands. The RD-41 antibody has been reported to block tetramer staining.

Applications Reported: This RD-41 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections and ELISA.

Applications Tested: This RD-41 antibody has been tested by flow cytometric analysis of dendritic cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Rae-1 delta is one of several known murine NKG2D ligands that include RAE-1 molecules, H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.