Thermo Fisher Scientific TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• APC (Cat. 17-5711-82)
• Biotin (Cat. 13-5711-82)
• Brilliant Violet 650 (Cat. 416-5711-82)
• eFluor 450 (Cat. 48-5711-82)
• FITC (Cat. 11-5711-82)
• PE (Cat. 12-5711-82)
• PE-Cyanine5 (Cat. 15-5711-82)
• PE-Cyanine7 (Cat. 25-5711-82)
• PerCP-eFluor 710 (Cat. 46-5711-82)
• Super Bright 645 (Cat. 64-5711-82)
• Super Bright 780 (Cat. 78-5711-82)
• Custom conjugation available upon request

Product Specific Information

The GL3 monoclonal antibody reacts with the gamma delta T-cell receptor complex (TCR) on all gamma delta TCR-bearing T cells, but does not react with alpha beta TCR. The gamma delta TCR is present in the thymus, epidermis, intestinal epithelium, peritoneal cavity, and lymphoid tissues.

Applications Reported: Flow cytometric analysis and immunoprecipitation.
Applications Tested: Tested by flow cytometric analysis of mouse lymph node cells. Recommended ≤0.25 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Titrate for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TCR gamma/delta (T-cell receptor γδ) are specialized T cells involved in immune recognition. These receptors discriminate foreign from self-peptides presented by MHC class II molecules, crucial for adaptive immunity. Abnormal TCR recognition is linked to autoimmune diseases and tumor-associated peptides. Gamma-delta T cells, mainly found in epithelial tissues, exhibit limited TCR diversity and play roles in antitumor and immunoregulatory responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.