Thermo Fisher Scientific Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), eBioscience

Thermo Fisher Scientific Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats:

• Biotin
• FITC
• Request custom conjugation

Product Specific Information

The Y-Ae antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules. It detects a determinant expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This determinant is found on peripheral B cells and thymic medulla cells, but not thymic cortical epithelium. It also appears on macrophages and dendritic cells, but not on invariant chain-associated class II MHC complexes.

The reactivity of Y-Ae antibody is MHC-restricted. It was generated by immunization of [B10.MBR x B10.D2]F1 recipients with B10A(5R) LPS blasts. The antibody reacts with 10–15% of surface I-Ab molecules in strains that also express surface I-Eb molecules (e.g., B10.A(5R), B10.A(3R)). It does not react with I-A molecules in strains with non-functional I-E (a chain) genes (e.g., B6, B10), mutant I-A molecules, or any other tested strain. The Y-Ae antibody specifically recognizes Ea peptide (Ea 52–68) bound to I-Ab, but not to Ea 52–68 bound to I-Ak or I-Abm-12.

Applications Reported:
This antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse strains expressing I-A(b)/MHC Class II:Ea peptide complex. Recommended ≤0.5 µg per test (100 µL final volume). Cell number may range from 10^5–10^8 cells/test. Titrate for optimal performance.

Purity: >90%, determined by SDS-PAGE
Aggregation: <10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

MHC class II molecules are heterodimers of non-covalently associated alpha (31–34 kDa) and beta (26–29 kDa) chains. Antigen presentation requires lysosomal proteases for peptide generation and removal of invariant chain (Ii) from the binding site. MHC class II molecules present exogenous antigens to CD4+ T lymphocytes. CD4 interacts with non-polymorphic residues of MHC class II.

HLA and MHC antibodies are crucial in Immunopeptidomics, enabling identification and characterization of neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.