Thermo Fisher Scientific CD93 (AA4.1) Monoclonal Antibody (AA4.1), FITC, eBioscience
마우스 C1qRp(CD93)를 인식하는 AA4.1 단클론 항체로, FITC 형광 표지되어 흐름세포분석에 적합합니다. 초기 B세포 발달 단계 및 조혈 전구세포 분석에 활용됩니다. 488 nm에서 여기, 520 nm에서 방출하며 4°C 암소 보관합니다.
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Applications
- Flow Cytometry (Flow): Tested at ≤ 1 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AA4.1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465297 |
Available Formats
- APC (Cat. 17-5892-82)
- Biotin (Cat. 13-5892-82)
- eFluor 450 (Cat. 48-5892-80)
- PE (Cat. 12-5892-82)
- PE-Cyanine7 (Cat. 25-5892-82)
- PerCP-Cyanine5.5 (Cat. 45-5892-82)
- Super Bright 436 (Cat. 62-5892-82)
- Super Bright 600 (Cat. 63-5892-82)
- Super Bright 702 (Cat. 67-5892-82)
- Request custom conjugation
Product Specific Information
The AA4.1 monoclonal antibody recognizes mouse C1qRp, a 130–140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen is expressed on early B cell developmental stages in bone marrow, as well as in adult spleen and fetal liver.
When combined with markers such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the AA4.1 antigen helps distinguish primitive and differentiated lymphoid progenitor subsets.
Expression of surface IgM, CD23, and AA4.1 defines three immature B cell subsets:
- T1: AA4.1⁺ / CD23⁻ / sIgM^hi
- T2: AA4.1⁺ / CD23⁺ / sIgM^hi
- T3: AA4.1⁺ / CD23⁺ / sIgM^lo
AA4.1 is also detected in endothelial cells, megakaryoblasts, and platelets. The AA4.1 and 493 antibodies recognize different epitopes of the same molecule.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow and splenocytes at ≤ 1 µg/test
Recommended Use: Optimize antibody titration for best performance
Excitation: 488 nm
Emission: 520 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The encoded protein is a type I membrane glycoprotein originally identified as a myeloid cell marker. It is implicated in intercellular adhesion and apoptotic cell clearance. The cytoplasmic tail interacts with moesin, linking it to cytoskeletal remodeling processes.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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