Thermo Fisher Scientific CD205 Monoclonal Antibody (205yekta), PE, eBioscience
Mouse CD205(DEC-205) 인식 단일클론 항체로, Flow Cytometry 분석에 최적화된 PE-conjugated 형식입니다. 항원 포획 및 제시 관련 연구에 적합하며, 0.03 µg/test로 사용 가능합니다. 4°C에서 암소 보관하며 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.03 µg/test
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 205yekta |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, Do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597429 |
Available Formats
- Alexa Fluor 488
- APC
- Biotin
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- NovaFluor Yellow 755
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The 205yekta antibody reacts with mouse CD205 (DEC-205), an integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation that enhances antigen-specific T cell immunity. CD205 is highly expressed by CD8⁺ dendritic cells and is also found in bone marrow Gr1⁺ cells, Langerhans cells, bone marrow-derived DCs, and thymic epithelial cells.
Note:
CD205 binds to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205⁺ dendritic cells.
Applications Reported:
Flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.03 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Optimize titration for best results.
Spectral Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD205 (LY75, Lymphocyte Antigen 75):
An integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation to enhance antigen-specific T cell immunity. Expressed in CD8⁺ dendritic cells, bone marrow Gr1⁺ cells, Langerhans cells, and thymic epithelial cells. Functions as an endocytic receptor directing captured antigens to specialized antigen processing regions and contributes to regulation of B lymphocyte proliferation.
Associated diseases: pneumonic plague, lipid pneumonia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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