Thermo Fisher Scientific Phospho-EGFR (Ser967) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-967) coupled to agarose, then affinity purified using phospho-EGFR (Ser-967) peptide (without carrier).
It detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment.

Target Information

EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors.
Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ERbB family of proteins (ERbB2, ERbB3, ERbB4).
Binding of ligands such as EGF and TGF-alpha to the extracellular domain leads to EGFR dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.