Thermo Fisher Scientific Phospho-EphA4 (Tyr779) Polyclonal Antibody
Phospho-EphA4 (Tyr779) 특이적 항체로 인간, 마우스, 랫트 시료에서 사용 가능. Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제된 rabbit polyclonal 항체. PBS/BSA/glycerol buffer에 보관되며 -20°C에서 안정적.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EphA4 (Tyr-779) synthetic peptide corresponding to amino acids surrounding phosphorylated tyrosine 779 in human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and is conserved well in most other EphA family members, but has less conservation in EphB family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942923 |
Product Specific Information
This antibody was cross-adsorbed to unrelated phosphotyrosine peptides before affinity purification using EphA4 (Tyr-779) peptide. The purified antibody detects a 120 kDa band corresponding to EphA4 in western blots of HUVEC cells treated with pervanadate.
Target Information
EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors are implicated in developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and two fibronectin type III repeats. The ephrin receptors are divided into two groups based on extracellular domain sequence similarity and their affinities for ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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