Thermo Fisher Scientific Phospho-EphA4 (Tyr602) Polyclonal Antibody
Phospho-EphA4 (Tyr602) 폴리클로날 항체로 인간, 생쥐, 랫트 시료에 반응합니다. Western blot 및 IHC(F) 분석에 적합하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. EphA4 인산화 상태 검출 연구용으로 최적화된 항체입니다.
- 카탈로그번호
- PA5143694
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EphA4 (Tyr-602) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding phosphorylated tyrosine 602 in human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and is conserved well in most other EphA and EphB family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942922 |
Product Specific Information
This antibody was cross-adsorbed to unrelated phosphotyrosine peptides before affinity purification using EphA4 (Tyr-602) peptide.
The purified antibody detects a 120 kDa* band corresponding to EphA4 in Western blots of HUVEC cells treated with pervanadate and in mouse brain tissue.
Target Information
EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family.
EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system.
Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and two fibronectin type III repeats.
The ephrin receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
EphA4 encodes a protein that binds ephrin-A ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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