Thermo Fisher Scientific Phospho-EGFR (Ser1142) Polyclonal Antibody
EGFR 단백질의 Ser1142 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal 항체입니다. Western blot 및 Immunocytochemistry에 사용 가능하며, 인간, 생쥐, 랫트 시료에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-EGFR (Ser-1142) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 1142 of human EGFR (ErbB-1). This human EGFR sequence has high homology with rat and mouse EGFR, and is not conserved in other ErbB family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942920 |
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-1142) coupled to agarose, then affinity purified using phospho-EGFR (Ser-1142) peptide (without carrier).
The antibody detects a ~180 kDa protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer, and EGFR is the target of both biological and small molecular therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ErbB family of proteins, which includes ERbB2, ERbB3, and ERbB4.
EGFR is a transmembrane receptor, and binding of its ligands such as EGF (Epidermal Growth Factor) and TGF-α (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach, and in squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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