Thermo Fisher Scientific Phospho-EGFR (Ser1142) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated EGFR (Ser-1142) coupled to agarose, then affinity purified using phospho-EGFR (Ser-1142) peptide (without carrier).
The antibody detects a ~180 kDa protein on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A, and does not detect this band after lambda phosphatase treatment.

Target Information

EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer, and EGFR is the target of both biological and small molecular therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ErbB family of proteins, which includes ERbB2, ERbB3, and ERbB4.
EGFR is a transmembrane receptor, and binding of its ligands such as EGF (Epidermal Growth Factor) and TGF-α (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach, and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.