Thermo Fisher Scientific Vinculin Monoclonal Antibody (7F9), Biotin, eBioscience
Human, Mouse, Rat 반응성의 비오틴 결합 단클론 항체로, Vinculin 및 Metavinculin을 인식합니다. 세포 접착 및 이동 관련 연구에 적합하며, ICC/IF, IHC 등 다양한 응용에 사용 가능합니다. 0.5 mg/mL 농도, PBS 버퍼, 4°C 보관.
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Thermo Fisher Scientific Vinculin Monoclonal Antibody (7F9), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 3 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7F9 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572839 |
Additional Formats
Product Specific Information
The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. It is involved in anchoring F-actin to the membrane and regulating E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Dysregulation of vinculin alters cell adhesion, migration, and growth, promoting cancer invasion and metastasis.
Applications Reported:
This 7F9 antibody has been reported for use in microscopy, immunocytochemistry, and immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections.
Applications Tested:
Tested by immunocytochemistry on formaldehyde-fixed and permeabilized cells; usable at ≤10 µg/mL. Antibody titration recommended for optimal performance.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It anchors F-actin to the membrane through interactions with talin, paxillin, and alpha-actinin. Vinculin plays a key role in the assembly of adhesion plaques and the linkage between the extracellular matrix and cytoplasmic microfilaments. Mutations in the VCL gene are associated with cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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