Thermo Fisher Scientific Amyloid Precursor Protein Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control for this product is murine brain lysate.

Target Information

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. Abeta peptide is 40–43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process.

The first step involves cleavage of the extracellular, amino-terminal domain of beta APP by beta-secretase (BACE), an aspartyl protease synthesized as a propeptide and activated by furin. This cleavage produces a secreted segment and a membrane-bound remnant. The remnant is further processed by gamma-secretase, a multi-subunit complex containing presenilin-1 and 2, which cleaves within the transmembrane domain to release the amyloid beta peptide.

Nicastrin, a transmembrane glycoprotein associated with presenilins, binds to the carboxyl-terminus of beta APP and modulates amyloid beta peptide production. Abeta forms extracellular filamentous protein deposits found in amyloid cores, neuritic plaques, and neurofibrillary tangles.

Alzheimer’s disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, Dutch-type amyloidosis, cerebral amyloid angiopathy, and the Guam Parkinson-Dementia complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.