Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Yellow 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
Monoclonal antibody HP-F1 recognizes CD85j (also known as ILT2, LILRB1, LIR1), a member of the ILT/LIR/MIR family. CD85j is a transmembrane glycoprotein with four ITIMs that signal via SHP-1. It is expressed on myeloid cells (monocytes, dendritic cells) and subsets of NK, T, and B cells. Expression correlates with leukemias such as ALL and CLL and plays roles in viral infections (HIV, EBV, CMV). Ligands include MHC Class I molecules (HLA-G, A, F, B27, E, F). HP-F1 reduces CD16-dependent cytolytic activity of NK cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells.
Use at 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures minimizing spillover.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Target Information

This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at 19q13.4. The encoded protein includes extracellular Ig domains, a transmembrane region, and ITIM motifs. It binds MHC class I molecules on antigen-presenting cells, transmitting inhibitory signals that modulate immune response and cytotoxicity. Multiple transcript variants exist.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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