Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Yellow 590, eBioscience

Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Yellow 590

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j (ILT2), also known as LILRB1 and LIR1.
CD85j is a transmembrane glycoprotein with a long cytoplasmic domain containing four ITIMs that signal through SHP-1.
Expression is observed on myeloid cells (monocytes, dendritic cells) and certain lymphoid cells (NK, T, B subsets). It correlates with leukemias (ALL, CLL) and viral infections (HIV, EBV, CMV).

Ligands for CD85j include MHC Class I molecules (HLA-G, A, F, B27, E, F).
HP-F1 antibody reduces CD16-dependent cytolytic activity of NK cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells.
Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume).

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for optimal results.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) should be added to antibody cocktails before combining with cells:

• Add 5 µL per sample (regardless of NovaFluor count)
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells)

NovaFluor conjugates utilize Phiton™ technology, engineered for minimal spillover and spectral spread.

Excitation: 552 nm
Emission: 592 nm
Laser: 561 nm (Yellow)

Target Information

The CD85j (LILRB1) gene is part of the leukocyte immunoglobulin-like receptor (LIR) family located on chromosome 19q13.4.
It encodes a receptor expressed on immune cells that binds MHC class I molecules and transduces inhibitory signals to modulate immune responses, inflammation, and cytotoxicity.
Multiple transcript variants encode different isoforms.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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