Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor Yellow 610

Applications

• Flow Cytometry

Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT/LIR/MIR family and is a single transmembrane glycoprotein with a long cytoplasmic domain containing four ITIMs that signal through SHP-1 interactions.

Expression is found on myeloid cells (monocytes and dendritic cells) and subsets of NK, T, and B lymphoid cells. It is correlated with leukemias such as ALL and CLL, and plays an important role in viral infections including HIV, Epstein-Barr, and CMV.

Ligands for CD85j include MHC Class I molecules such as HLA-G, A, F, B27, E, and F. HP-F1 antibody has been shown to reduce CD16-dependent cytolytic activity of NK cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg)/test (in 100 µL final volume).

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which provides improved formulation. Add 5 µL per sample for optimal staining. For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, featuring engineered fluorescent signatures designed to minimize spillover and spread impacts.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Target Information

The CD85j gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at chromosome region 19q13.4. It encodes a receptor with two to four extracellular immunoglobulin domains, a transmembrane domain, and multiple ITIMs. This receptor is expressed on immune cells, binds MHC class I molecules, and transduces inhibitory signals to regulate immune responses, inflammation, and cytotoxicity. Multiple transcript variants exist for this gene.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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