Thermo Fisher Scientific Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody
ERK1/2의 Tyr204 인산화를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot과 IHC(P)에서 사용 가능하며, 세포 내 신호전달 연구에 적합. 액상 형태로 제공되며, -20°C에서 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAPK3 (Accession P27361), corresponding to amino acid residues around phosphorylated Tyr204. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816027 |
Product Specific Information
This antibody detects endogenous levels of ERK1/2 only when phosphorylated at Tyrosine 204.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric G protein-coupled receptors, and transforming agents, activate the ERK1/2 pathways.
When growth factors bind to receptor tyrosine kinases, Ras interacts with Raf and activates it. Activated Raf phosphorylates MEK1/2, which in turn phosphorylates ERK1/2.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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