Thermo Fisher Scientific Phospho-Cyclin D1 (Thr286) Polyclonal Antibody
Cyclin D1의 Thr286 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC에 사용 가능. Human, Mouse, Rat 반응성. 1 mg/mL 액상 형태로 -20°C 보관. 세포주기 및 암 연구용에 적합.
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- PA5104551
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CCND1 (Accession P24385), corresponding to amino acid residues around phosphorylated Thr286. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816026 |
Product Specific Information
This antibody detects endogenous levels of Cyclin D1 only when phosphorylated at Threonine 286.
Target Information
Cyclin D1 (also known as PRAD-1 or bcl-1) is a key regulator of the cell cycle that functions with CDK4 and/or CDK6 to phosphorylate the Rb protein. It acts as a proto-oncogene and is overexpressed in various human cancers, including mantle cell lymphoma (MCL). Cyclin D1 regulates protein phosphorylation, epithelial cell proliferation, and adipocyte differentiation. The CCND1 gene is located on chromosome 11 and encodes a cytoplasmic and nuclear protein synthesized during G1 phase. Cyclin D1 forms complexes with CDK4 or CDK6, promoting G1/S transition by inactivating Rb and modulating CDK inhibitors (p21Cip1, p27Kip1). Phosphorylation triggers its ubiquitin-mediated degradation, leading to G1/S arrest. Due to its critical role in cell cycle control, Cyclin D1 is a valuable marker and research target in oncology and cell biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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