Thermo Fisher Scientific AKT Pan Polyclonal Antibody
AKT1/2/3 단백질의 내인성 발현을 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며, 고순도의 Affinity chromatography로 정제됨. PBS/glycerol buffer에 보관하며 -20°C에서 안정적 저장 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AKT1 (Accession P31749), corresponding to amino acid residues G410–C460 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2853848 |
Product Specific Information
This antibody detects endogenous levels of total pan-AKT1/2/3.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a ubiquitous serine/threonine kinase involved in regulating metabolism, cell survival, and growth by phosphorylating multiple proteins. It is activated by insulin, PI3K, IGF1, and other growth and survival factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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