Thermo Fisher Scientific TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), Biotin, eBioscience
인간 배아줄기세포 및 암세포에서 발현되는 Podocalyxin을 인식하는 단클론 항체입니다. Biotin 결합형으로 Flow Cytometry에 최적화되어 있으며, 0.25 µg/test로 사용 가능합니다. 분화 과정에서 소실되는 줄기세포 표지자 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 13 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TRA-1-60 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891594 |
Additional Formats
Product Specific Information
The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonal carcinoma cells (EC), and embryonic germ cells (EG). The epitope recognized by this antibody is lost upon cell differentiation. Contrary to early reports, TRA-1-60 recognizes a neuraminidase-resistant antigen.
TRA-1-60 specifically binds to a carbohydrate epitope on podocalyxin, a keratan-sulfated glycoprotein and member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on glomerular epithelial cells (podocytes) and has been associated with cancer aggressiveness in breast and prostate cancers.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on human embryonal carcinoma (EC) line 2102Ep. Recommended ≤0.25 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TRA-1-60 is a cell surface antigen expressed along with SSEA-3, SSEA-4, and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPSCs). These markers are lost during differentiation, whereas SSEA-1 appears after retinoic acid-mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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