Thermo Fisher Scientific Neurofilament Recombinant Mouse Monoclonal Antibody (rNF421)
Neurofilament 단백질 검출용 Mouse 단클론 항체로, Western blot, IHC, Flow cytometry 등 다양한 응용에 적합. HEK293 세포 발현 시스템 기반의 재조합 항체이며, NF-H 단백질에 특이적. 고순도 Protein A/G 정제, PBS 버퍼에 안정화되어 연구용으로 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rNF421 |
| Immunogen | Recombinant full-length human NEFH protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Neurofilaments are intermediate filament proteins specific to neurons, composed mainly of NF-L, NF-M, and NF-H. Expression of these proteins is tissue-specific and developmentally regulated. NF-H plays an essential role in mature axons and is implicated in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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