Thermo Fisher Scientific Neurofilament Recombinant Rabbit Monoclonal Antibody (NEFL.H, 2324R)
Neurofilament Heavy Chain(NF-H)을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, Flow Cytometry에 사용 가능. 인간, 생쥐, 닭, 돼지, 랫트 반응성. HEK293 발현, Protein A/G 정제. 신경세포 축삭 유지 연구용에 적합.
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Thermo Fisher Scientific Neurofilament Recombinant Rabbit Monoclonal Antibody (NEFL.H, 2324R)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | NEFL.H, 2324R |
| Immunogen | Recombinant full-length human NEFH protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Neurofilaments are intermediate filament proteins specifically found in neurons and composed of three major proteins: NF-L, NF-M, and NF-H.
NF-H plays a crucial role in maintaining neuronal caliber and is involved in amyotrophic lateral sclerosis (ALS).
Expression of neuronal intermediate filament proteins is tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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