Thermo Fisher Scientific Phospho-NF-H Monoclonal Antibody (NE14)
Phospho-NF-H 단백질을 인식하는 NE14 클론의 마우스 모노클로날 항체로, 신경세포 내 신경섬유 유지 연구에 적합. Western blot, IHC, Flow cytometry 등 다양한 응용 가능. ALS 관련 연구 및 신경세포 발달 연구에 활용.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Chicken, Cat, Gerbil, Guinea pig, Human, Mouse, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NE14 |
| Immunogen | Crude neurofilament preparation from porcine spinal cord |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons, composed predominantly of three major proteins: NF-L, NF-M, and NF-H. These proteins are tissue-specific and developmentally regulated. NF-H plays a crucial role in maintaining mature axon structure and is implicated in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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