Thermo Fisher Scientific Cdc37 Monoclonal Antibody (C1)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA3-029 detects Cdc37 from hamster, human, mouse, and rat cells and tissues.
It has been successfully used in Western blot, FACS, immunofluorescence, immunocytochemistry, and immunoprecipitation (under denaturing conditions).

• In Western blot, this antibody recognizes a single 50 kDa protein representing Cdc37 in HeLa cell extract.
• Immunofluorescence staining of Cdc37 in Hepa1 cells with MA3-029 shows mostly cytoplasmic staining concentrated around the nuclear membrane.
• Weak non-specific bands may appear in Jurkat, A431, and NIH-3T3 cell lysates.

The MA3-029 antigen is purified mouse Cdc37 fusion protein.

Target Information

In S. cerevisiae, Cdc37 is a key regulator of the cell division cycle through its action on cyclin-dependent kinases (CDKs).
Cdc37 is also associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase, and the sevenless receptor tyrosine kinase.

HSP90 has been shown to interact with many of the same cellular targets as Cdc37. Cdc37 (also known as p50) forms complexes with Src-family kinases (Fyn, Yes, Fes, Lck, Fgr), Raf, and pp60v-src.
Cdc37 functions as a chaperone both independently and in concert with HSP90, and is necessary for the binding of HSP90 to Cdk4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.