Thermo Fisher Scientific GAL4 Monoclonal Antibody (2GV-3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Yeast

Host/Isotype

Mouse / IgG, kappa

Class

Monoclonal

Type

Antibody

Clone

2GV-3

Immunogen

Gal4 DNA Binding Domain aa1-147

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2608454

Product Specific Information

MA3-031 recognizes Gal4 DNA Binding Domain corresponding to amino acids 1-147 of the GAL4 DNA binding region.

Target Information

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.