Thermo Fisher Scientific HCN2 Polyclonal Antibody
HCN2 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고순도 항체.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic Peptide: G(874)LD PQDSARSRLSSNL(889) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2115010 |
Product Specific Information
PA1-918 detects HCN2 from rat samples.
Successfully used in Western blot procedures.
By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated forms of HCN2 in rat brain extract.
The immunizing peptide corresponds to amino acid residues 874–889 from human HCN2.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.
HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides.
HCN1 and HCN2 play important roles in motor learning and neuronal integration by cerebellar Purkinje cells, as well as shaping autonomous activity of single neurons and periodicity of network oscillations.
HCN1 expression is enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus.
HCN2 is abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body.
These regional variations in HCN channel expression may cause differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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