Thermo Fisher Scientific alpha-ENaC Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Gerbil, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues L(20) M K G N K R E E Q G L G P E P A A P Q Q P T(42) C of Human alpha-Enac. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha-ENaC,uniProtId:P37088-1,ncbiNodeId:9606,antigenRange:20-42,antigenLength:669,antigenImageFileName:PA1-920A_alpha-ENaC_P37088-1_Rabbit.svg,antigenImageFileNamePDP:PA1-920A_alpha-ENaC_P37088-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2184369

Product Specific Information

PA1-920A detects alpha-epithelial sodium channel (alpha-ENaC) from human, mouse, and rat tissues and cells.

PA1-920A has been successfully used in Western blot procedures. By Western blot, this antibody specifically detects a ~97 kDa band representing glycosylated alpha-ENaC from NIH-3T3 cells, and a ~75 kDa band representing the unglycosylated alpha-ENaC protein from human brain samples.

The PA1-920A immunizing peptide is a synthetic peptide corresponding to residues 20-42 from human alpha-ENaC. This sequence is 82%, 73%, 64%, and 59% conserved in the rat and rabbit, mouse, guinea pig, and cow, respectively. PA1-920A immunizing peptide (Cat. # PEP-088) is available for use in neutralization and control experiments.

Target Information

Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel`s syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.