Thermo Fisher Scientific ATG4D Polyclonal Antibody
ATG4D 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 IHC에 사용 가능. 사람과 생쥐 시료에 반응하며, 자가포식 관련 단백질 연구에 적합. 동결건조 형태로 제공되며, 장기 보관 시 -20°C에서 안정적.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:200–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human ATG4D conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in mouse.
- Specificity: ATG4D.
Target Information
Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid cytoplasmic protein belonging to the C-54 family of cysteine proteases. It is predominantly expressed in skeletal muscle and testis. Atg4D acts as a cysteine protease required for autophagy, cleaving the C-terminal region of target proteins to enable binding to autophagosomes. Its enzymatic activity is inhibited by N-ethylmaleimide, a thiol-reactive compound that modifies cystine residues in proteins and peptides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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