Thermo Fisher Scientific ATG4B Polyclonal Antibody
ATG4B 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western Blot 및 Immunohistochemistry에 적합. 인간과 마우스 반응성 확인. 동결건조 형태로 제공되며, 장기 보관 시 -20°C에서 안정적 유지.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:200–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 20–80 of human ATG4B conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The antigen is homologous in mouse.
- Specificity: ATG4B.
Target Information
Cysteine protease ATG4B is an enzyme coded by the ATG4B gene. Autophagy is a critical cellular homeostatic process that controls turnover of damaged organelles and proteins. ATG4B acts as a deubiquitin-like protease playing dual roles in autophagy machinery. It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, and GABARAP) to expose a C-terminal glycine, essential for conjugation to phosphatidylethanolamine (PE) and membrane insertion required for autophagy. ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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