Thermo Fisher Scientific NRF1 Recombinant Rabbit Monoclonal Antibody (JM89-63)
NRF1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 높은 특이성과 민감도를 제공하며, 로트 간 일관성과 동물 유래 성분이 없는 제형으로 연구용에 최적화되어 있습니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:1,000 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM89-63 |
| Immunogen | Synthetic peptide within Human NRF1 aa 333–382 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810059 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits and screen individual clones to select optimal production candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
NRF1 encodes a transcription factor that homodimerizes and activates key metabolic genes involved in cellular growth, respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It is also associated with neurite outgrowth regulation.
Alternate splice variants encoding the same or different isoforms have been described, though not fully characterized. Note that confusion may occur in literature due to the shared symbol “NRF1” with nuclear factor (erythroid-derived 2)-like 1 (NFE2L1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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