Thermo Fisher Scientific TFF1 Recombinant Rabbit Monoclonal Antibody (JM37-86)
TFF1 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP에 사용 가능. 높은 특이성과 재현성을 제공하며, Protein A로 정제된 액상 형태. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM37-86 |
| Immunogen | Synthetic peptide within Human Estrogen Inducible Protein pS2 (aa 35–84) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810058 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and expressed, followed by screening to select optimal clones for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Trefoil family members possess one or more trefoil motifs—40 amino acid domains containing three conserved disulfide bonds. These are stable secretory proteins expressed in gastrointestinal mucosa and may protect the mucosa, stabilize mucus layers, and promote epithelial healing.
TFF1 is expressed in gastric mucosa and has been studied for its expression in human tumors. TFF1 and related genes are located in a cluster on chromosome 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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