Thermo Fisher Scientific RAPGEF3 Recombinant Rabbit Monoclonal Antibody (JM63-29)
RAPGEF3 단백질을 인식하는 재조합 토끼 단일클론 항체로 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. HEK293 세포에서 발현되며 lot 간 일관성과 동물 유래 성분이 없는 포뮬레이션 특징.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM63-29 |
| Immunogen | Synthetic peptide within Human Epac1 (aa 874–923) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2810053 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Gene ontology (GO) annotation includes small GTPase-mediated signal transduction.
The target protein binds cAMP and activates the Ras superfamily guanine nucleotide binding protein (Rap1A) in a PKA-independent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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