Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI2B1), TrueMAB
UBE2E3 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot과 유세포분석에 적합합니다. 동결건조 형태로 제공되며, PBS 버퍼(8% trehalose) 내에서 -20°C 보관 권장. 단백질 유비퀴틴화 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B1 |
| Immunogen | Full length human recombinant protein of human UBE2E3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.
UBE2E3 (also known as UBCH9) catalyzes the covalent attachment of ubiquitin to other proteins and participates in regulating transepithelial sodium transport in renal cells.
It may also play a role in cell growth arrest. UBE2E3 shuttles between the cytoplasm and nucleus in an IPO11-dependent manner. It is ubiquitously expressed at low levels and is highly expressed in skeletal muscle.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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