Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI1A3), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

The ubiquitin (Ub) pathway involves three enzymatic steps enabling conjugation of Ub and Ub-like molecules to specific substrates:

1. Activation: ATP-dependent activation of Ub C-terminus by E1 enzyme.
2. Conjugation: Formation of Ub-E2 intermediate complex by E2 enzyme.
3. Ligation: Transfer of Ub from E2 to substrate catalyzed by E3 ligase.

UBE2E3 (also known as UBCH9) participates in ubiquitin attachment to proteins, regulating transepithelial sodium transport in renal cells and possibly cell growth arrest.
It shuttles between cytoplasm and nucleus in an IPO11-dependent manner and is ubiquitously expressed, with high levels in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.