Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI1D10), TrueMAB

Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI1D10), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.

1. Activation: The Ub C-terminus is activated in an ATP-dependent manner by the E1 enzyme.
2. Conjugation: The activated Ub is transferred to the E2 enzyme, forming a Ub-E2 complex.
3. Ligation: The E3 ligase catalyzes the transfer of Ub from E2 to the target substrate.

UBE2E3 (also known as UBCH9) catalyzes the covalent attachment of ubiquitin to other proteins and plays a role in regulating transepithelial sodium transport in renal cells. It may also be involved in cell growth arrest.
UBE2E3 shuttles between the cytoplasm and nucleus in an IPO11-dependent manner and is expressed ubiquitously at low levels, with high expression in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.