Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI4G4), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.

1. Activation: ATP-dependent activation of the Ub C-terminus by the E1 enzyme.
2. Conjugation: Transfer of Ub to the Ub-conjugating enzyme (E2) forming a Ub–E2 complex.
3. Ligation: The Ub-ligase (E3) catalyzes the transfer of Ub from E2 to the target protein.

UBE2E3 (also known as UBCH9) catalyzes ubiquitin attachment to target proteins and regulates transepithelial sodium transport in renal cells. It may also play a role in cell growth arrest. The UBE2E3 protein shuttles between cytoplasm and nucleus in an IPO11-dependent manner and is expressed ubiquitously, with high expression in skeletal muscle.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.