Thermo Fisher Scientific CD117 (c-kit) Polyclonal Antibody
CD117(c-kit) 폴리클로날 항체로 인간, 마우스, 랫트 시료에 반응합니다. IHC, WB, Flow Cytometry 등 다양한 응용에 사용 가능하며, Jurkat 세포를 양성 대조로 활용할 수 있습니다. 액상 형태로 제공되며, 항원 친화 크로마토그래피로 정제되었습니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD117 (c-kit) Polyclonal Antibody
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–1 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from N-terminus of Kit protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Jurkat cells
- Cellular location: Membrane, cytoplasmic
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for mast cell growth factor (MGF, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. Together with its ligand, KIT regulates growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of KIT receptor tyrosine kinase, particularly by the D816V activation loop mutation, is a key therapeutic target in neoplastic mast cell growth.
c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon binding stem cell factor (SCF), c-kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues such as Tyr703. This enables Grb2 docking and activation of the Ras/ERK pathway. SCF/c-kit signaling activates multiple downstream pathways including PI3K, PLC-γ, and JAK/STAT, essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.