Thermo Fisher Scientific Kinesin 5B Monoclonal Antibody (KN-02)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody recognizes the heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity for denatured molecules. The epitope is located in the coiled-coil stalk domain and stains Western blots of kinesin-enriched preparations.
Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02, and KN-03 react with different sets of fragments.
The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.

Target Information

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. It can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner.
Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, it antagonizes dynein function and drives the separation of nuclei and centrosomes.
Required for anterograde axonal transportation of MAPK8IP3/JIP3, which is essential for MAPK8IP3/JIP3 function in axon elongation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.