Thermo Fisher Scientific NOTCH1 Chimeric Recombinant Rabbit Monoclonal Antibody (E6)
NOTCH1 단백질을 표적하는 재조합 토끼 단클론 항체로, 인간 및 생쥐 반응성 보유. IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 실험에 사용 가능. 높은 특이성과 안정적인 액상 형태로 제공되며, 단백질 A로 정제됨.
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Thermo Fisher Scientific NOTCH1 Chimeric Recombinant Rabbit Monoclonal Antibody (E6)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, lambda |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | E6 |
| Immunogen | Fusion protein consisting of EGF domains 1-12 of murine Notch1 fused to a human Fc domain; binds to the NRR of mouse Notch1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3074265 |
Product Specific Information
- Specificity: Engineered ScFv fragment antibody that binds to both mouse mNRR1 and human hNRR1 but not NRR2.
- Preparation: Selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to the NRR of mouse Notch1.
Target Information
The Notch gene family encodes transmembrane proteins with multiple cysteine-rich EGF-like repeats in the extracellular region. Four mammalian Notch genes (Notch1, Notch2, Notch3, Notch4/Int-3) are involved in differentiation of the nervous system and other structures.
EGF-like proteins Delta and Serrate act as ligands for Notch1. Mature Notch proteins are heterodimeric receptors produced by cleavage of precursor Notch proteins into an extracellular subunit (NEC) and a transmembrane subunit (Ntm) containing intracellular regions.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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