Thermo Fisher Scientific NOTCH1 Chimeric Recombinant Mouse Monoclonal Antibody (E6)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, lambda

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

E6

Immunogen

This antibody was selected against a fusion protein consisting of EGF domains 1-12 of murine Notch1 fused to a human Fc domain and binds to the NRR of mouse Notch1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074264

Product Specific Information

Specificity: An engineered ScFv fragment antibody that binds to both mouse mNRR1 and human hNRR1 but not NRR2.

Preparation: This antibody was selected against a fusion protein consisting of EGF domains 1-12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1.

Target Information

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.