Thermo Fisher Scientific Phospho-c-Fos (Ser362) Polyclonal Antibody
Phospho-c-Fos (Ser362) 단백질을 인식하는 Rabbit Polyclonal 항체로, WB와 IHC(P) 실험에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 1 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Serine 362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2553716 |
Product Specific Information
- Detects endogenous protein at a molecular weight of approximately 62 kDa.
- Purity is greater than 95% by SDS-PAGE.
Target Information
FBJ murine osteosarcoma viral oncogene homolog, commonly known as Cellular proto-oncogene Fos (c-Fos), belongs to the Fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14.
c-Fos heterodimerizes with c-Jun to form the Activator Protein-1 (AP-1) transcription factor, which regulates transcription of several genes involved in cellular signal transduction, proliferation, and differentiation.
Phosphorylation of c-Fos occurs at multiple sites in response to stimuli such as growth factors and insulin. The best-characterized kinase for phosphorylation at Threonine 232 is ERK MAPK, which regulates c-Fos nuclear localization and transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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