
Thermo Fisher Scientific RFP Polyclonal Antibody
RFP 및 다양한 변형체(mCherry, tdTomato 등)에 반응하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 고순도 Affinity chromatography 정제, 안정적인 장기 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Red Fluorescent Protein (RFP) fusion protein (234aa) derived from Discosoma coral |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.01% BSA, 0.15M NaCl, 25% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 2–8°C prior to opening.
- May aliquot and freeze at -20°C or below for extended storage.
- Avoid freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Dilute only prior to use.
Expected Reactivity:
RFP and its variants including mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry.
Immunoelectrophoresis shows a single precipitin arc against anti-Rabbit Serum and purified RFP (Discosoma).
No cross-reactivity observed with Human, Mouse, or Rat serum proteins.
Target Information
Red Fluorescent Protein (RFP) is isolated from Discosoma sp. (Uniprot ID: Q9U6Y8).
Commonly used as a molecular tag for studying:
- Protein localization
- Reporter gene expression
- Protein-protein interactions
RFP exists as a homotetramer, but monomeric variants are available for various applications.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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