Thermo Fisher Scientific 6X His Epitope Tag Polyclonal Antibody
6X His epito프 태그 단백질 검출용 폴리클로날 항체로, Western blot, ELISA, IHC, IP에 적합. Rabbit IgG 기반 비결합형 액상 항체이며, 높은 특이성과 낮은 배경 반응을 제공. His-tag 단백질의 정량 및 확인에 유용.
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Thermo Fisher Scientific 6X His Epitope Tag Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:10,000–1:40,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified from whole rabbit serum immunized with 6X His epitope tag peptide (H-H-H-H-H-H) conjugated to KLH using maleimide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. It detects overexpressed proteins containing the 6X His epitope tag and has shown reactivity with all His-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Recognizes His-tag (His-His-His-His-His-His) fused to either amino or carboxy termini of targeted proteins in transfected or transformed cells.
Target Information
The 6X His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins for which no specific antibody exists. His-tagged proteins are purified using metal chelate chromatography due to the strong affinity of histidine residues for metal ions such as Ni²⁺.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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