Thermo Fisher Scientific SPTBN1 Monoclonal Antibody (4C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4C3

Immunogen

Purified human erythrocyte beta-1 spectrin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPTBN1,uniProtId:Q01082-1,ncbiNodeId:9606,antigenRange:1-2364,antigenLength:2364,antigenImageFileName:MA3-062_SPTBN1_Q01082-1_House_mouse.svg,antigenImageFileNamePDP:MA3-062_SPTBN1_Q01082-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2194360

Product Specific Information

MA3-062 detects spectrin from mouse, rat and human erythrocytes, brain and muscle cells. This antibody has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits.

MA3-062 has been successfully used in Western blot, immunohistochemistry (paraffin) and immunofluorescence procedures. By Western blot, this antibody detects a ~246 kDa protein representing beta-1 spectrin in rat skeletal muscle homogenate. Immunofluorescence staining of beta-1 spectrin in rat skeletal muscle fibers with MA3-062 results in intense staining of the sarcolemma.

In immunofluorescence, antigen integrity can be compromised if aldehyde fixatives are left in contact with the protein for extended periods of time. If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution.

The MA3-062 antigen is purified human erythrocyte beta-1 spectrin.

Target Information

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e. g. beta-1 epsilon-1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.