Thermo Fisher Scientific CLEC11A Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
GHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ AAAQARCTAR GGSLAQPADR QQMEALTRYL RAALAPYNWP VWLGVHDRRA EGLYLFENGQ RVSFFAWHRS PRPELGAQPS ASPHPLSPDQ PNGGTLENCV AQASDDGSWW DHDCQRRLYY VCEFPF

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human SCGF-alpha. Anti-Human SCGF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human SCGF-alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCGF-alpha (500-P162) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human SCGF-alpha.

Western Blot:
To detect Human SCGF-alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human SCGF-alpha is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P162BT-1 mg will be provided as 2 × 500 µg.

Target Information

This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.